MICROBIOLOGIA

BIOSEGURIDAD EN LABORATORIOS DE
MICROBIOLOGÍA Y BIOMEDICINA
TRADUCCIÓN AL ESPAÑOL

Ministerio de Salud
Subsecretaria de Programas
de Prevención y Promoción

Programa de Vigilancia de la Salud
y Control de Enfermedades

Administración Nacional de Laboratorios
e Institutos de Salud

GRUPO REVISOR
Raquel Terragno
Angel José Cingara
Elsa Graciela Bauminster
Liliana BeatrizLatapie
Ana María Ambrosio
Ana María de Risso
Osvaldo Rico Cordeiro

Química.
Médico Veterinario.
Bioquímica.
Química.
Bioquímica.
Bioquímica
Epidemiólogo.

INEI “Dr. Carlos G. Malbrán”
INP “Dr. Mario Fatala Chaben”
INEI “Dr. Carlos G. Malbrán”
INEI “Dr. Carlos G. Malbrán”
INEVH “Dr. Julio Maiztegui”
INP “Dr. Mario Fatala Chaben”
Vigilancia de la Salud. VIGI+A

EDITORESJonathan Y. Richmond, Ph. D.
Director, Office of Health and Safety
Public Health Service
Centers for Disease Control and Prevention
1600 Clifton Road N.E.
Atlanta, Georgia 30333

Robert W. McKinney, PH.D.
Director, Division of Safety
Public Health Service
National Institutes of Health
Building 31, Room 1C02
Bethesda Maryland 20892

I

EDITORES INVITADOS

Centers for DiseaseControl Prevention

Robert B. Craven, M.D.

Chief, Epidemiology Section
Arbovirus Disease Branch
National Center for Infectious
Diseases

Mark L. Eberhard, Ph.D.

Chief
Biology and Diagnostics Branch
Division of Parasitic Diseases
National Center for Infectious
Diseases

Thomas Folks, Ph.D.

Chief
HIV and Retroviroly Branch
Division of AIDS, STD, and
Laboratory ResearchNational Center for Infectious
Diseases

Bradford Kay, Dr. P.H.

Senior Laboratory Advisor
Division of Bacteriology and
Mycotic Diseases
National Center Infectious
Diseases

Richard C. Knudsen, Ph. D.

Chief
Labboratory Safety Branch
Office of Health and Safety

Brian W.J. Mahy, Sc.D.,
Ph.D.

Director
Division of Viral and Rickettsial
Diseases
National Center for InfectiousDiseases

C.J. Peters, M.D.

Chief
Special Pathogens Branch
National Center for Infectious
Diseases

Margaret A. Tipple, M.D.

Chief
External Activities Program
Office of Health and Safety

II

John Bennett, M.D.

Chief, Micology Section
National Institute for Allergies
and Infectious Diseases

David Hackstadt, PH.D.

Microbiologist
Rocky Mountain LaboratoryDeborah E. Wilson, Dr. P.H.

Chief, Occupational Health and
Safety Branch
Division of Safety

EDITORES INVITADOS INDIVIDUALES
Jonathan Crane, A.I.A.

Architect
Atlanta, GA

Peter J. Gerone, Sc.D.

Director, Tulane Regional
Primate
Research Center
Tulane University Medical
Center
Covington, Louisiana

Thomas Hamm, D.V.M.
Ph.D.

Consultant
Cary, NC

Debra L. Hunt, Dr. P.H.Director, Biological Safety and
Infection Control
Duke University Medical Center
Durham, North Carolina

Peter Jahrling, Ph.D.

Senior Research Scientist
Disease Assessment Division
USAMRIID
Frederick, Maryland

Thomas Kost, Ph.D.

Section Head
Molecular Sciences Department
Glaxo Welcome, Inc.
Research Triangle Park, NC

EDITOR TECNICO
Marie J. Murray

Writer-EditorAtlanta, GA

III

ÍNDICE

SECCIÓN I
Introducción..............................................................................................1
SECCIÓN II
Principios de Bioseguridad.......................................................................5
SECCIÓN III
Criterios de Nivel de Bioseguridad de los Laboratorios.......................12
Tabla 1. Resumen de los Niveles deBioseguridad
Recomendados para Agentes Infecciosos.......................37
SECCIÓN IV
Criterios de Nivel de Bioseguridad de los Animales Vertebrados...........38
Tabla 1. Resumen de los Niveles de Bioseguridad
Recomendados para las Actividades en
las cuales se utilizan Animales Vertebrados
Infectados Natural o Experimentalmente........................55
SECCIÓN V
Evaluación de...