06 Vacunacion

Sitio Argentino de Producción Animal

Artículos científicos
La vacunación simultánea de bovinos con Lactobacillus
casei y la vacuna bivalente contra babesiosis bovina
genera una mejor protección contra Babesia bovis
y B. bigemina transmitidas por garrapatas
en condiciones extremas de campo
The simultaneous vaccination of bovines
with Lactobacillus casei and the bivalent vaccine
against bovinebabesiosis induces a better protection
against Babesia bovis and B. bigemina transmitted
by ticks in extreme field conditions
Carlos R. Bautista-Garfias*
Carmen Rojas-Martínez*

Roberto Castañeda-Arriola* Jesús A. Álvarez-Martínez*
Julio V. Figueroa-Millán* Astrid Rodríguez-Lozano*,**

Abstract
The effect of Lactobacillus casei on INIFAP’s mixed vaccine against bovine babesiosis (VAC) was assessed inbovines in an
endemic babesiosis area. It was previously reported that L. casei increases the efficiency of the Mexican mixed vaccine against
bovine babesiosis under controlled conditions. The results of the present study demonstrated the effectiveness of simultaneous
vaccination of bovines with L. casei and the mixed vaccine against bovine babesiosis in eliciting a protective immune responseunder extreme conditions in the field. Twenty Babesia spp free bovines were allocated into three groups: un-vaccinated (Control, n = 9), vaccinated with VAC (n = 5), and vaccinated simultaneously with VAC and Lactobacillus casei (LC-VAC, n = 6). All
animals were kept in a tick and Babesia spp free field at Coatepec, Veracruz during 24 days before moving them to Paso del
Toro, Veracruz, for a naturalexposition to Babesia spp transmitted by Riphicephalus (Boophilus) microplus ticks. Protection
against Babesia spp was observed in bovines belonging to VAC and LC-VAC groups, while control animals showed severe
clinical babesiosis. Bovines in VAC-LC group showed less clinical signs between days 12-16 after challenge as compared with
animals in VAC group. All bovines showed both Babesia spp afterchallenge. Levels of IgG anti-Babesia in animals from both
vaccinated groups, determined by indirect immunofluorescence test, always were higher to Babesia bovis than to B. bigemina
after vaccination and challenge. It was demonstrated the efficiency of simultaneous vaccination of bovines with VAC and L. casei, in eliciting a better protective immune response against naturally transmitted Babesiaspp under extreme field conditions.

Key words: BOVINE BABESIOSIS BIVALENT VACCINE, Lactobacillus
LLENGE.

casei,

EFFICACY, FIELD CHA-

Resumen
Se evaluó el efecto de Lactobacillus casei en la vacuna mixta contra babesiosis bovina del INIFAP (VAC), en bovinos
de un área endémica de babesiosis. Previamente se informó que L. casei incrementa la eficacia de la vacuna mixta mexicana contra babesiosisbovina bajo condiciones controladas. Los resultados aquí expuestos demostraron dicha
efectividad para generar una respuesta inmunitaria protectora bajo condiciones extremas en el campo. Veinte bovinos
libres de Babesia spp fueron distribuidos al azar en tres grupos: testigo no vacunado (Testigo, n = 9), vacunado con VAC
(n = 5), y vacunado simultáneamente con VAC y L. casei (LC-VAC, n = 6). Todoslos animales se mantuvieron en un corral libre de garrapatas y Babesia spp en Coatepec, Veracruz durante 24 días antes de transportarlos a Paso del Toro,
Veracruz, para una exposición natural a Babesia spp transmitida por garrapatas Riphicephalus (Boophilus). Se observó
protección contra Babesia spp en bovinos pertenecientes a los grupos VAC y LC-VAC, mientras que los animales testigo

Recibido el22 de agosto de 2011 y aceptado el 27 de febrero de 2012.
*Centro Nacional de Investigación Disciplinaria en Parasitología Veterinaria, Instituto Nacional de Investigaciones Forestales, Agrícolas y
Pecuarias, carretera Federal Cuernavaca-Cuautla 8534, col. Progreso, Jiutepec, 62550, Morelos, México.
**C.E.P. La Posta, km 22.4, carretera Veracruz-Córdoba, Paso del Toro, Medellín de Bravo, 91700,...