Analisis De Aguas

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Standard Methods for the Examination of Water and Wastewater 20th Edition
© Copyright 1999 by American Public Health Association, American Water Works Association, Water EnvironmentFederation
Test Condition Acute Test Survival and Growth
Testacceptability Mortality of control organisms≤10% Mortality of control
average dry wei
organism in con
≥0.25 mg


Part 9000 MICROBIOLOGICAL EXAMINATION
9010INTRODUCTION*#(560)

The following sections describe procedures for making microbiological examinations of water samples to determine sanitary quality. The methods are intended to indicate the degreeof contamination with wastes. They are the best techniques currently available; however, theirlimitations must be understood thoroughly.Tests for detection and enumeration of indicator organisms, rather than ofpathogens, areused. The coliform group of bacteria, as herein defined, is the principal indicator of suitabilityof a water for domestic, industrial, or other uses.

The cultural reactions and characteristics of this group of bacteria have been studied extensively.Experience has established the significance of coliform group density as a criterion of thedegree of pollution and thus of sanitaryquality. The significance of the tests and theinterpretation of results are well authenticated and have been used as a basis for standards of bacteriological quality of water supplies.The membrane filter technique, which involves a direct plating for detection and estimationof coliform densities, is as effective as the multiple-tube fermentation test for detectingbacteria of the coliform group.Modification of procedural details, particularly of the culturemedium, has made the results comparable with those given by the multiple-tube fermentationprocedure. Although there are limitations in the application of the membrane filter technique,it is equivalent when used with strict adherence to these limitations and to the specifiedtechnical details. Thus, two standard methods are presented for thedetection and enumerationof bacteria of the coliform group.It is customary to report results of the coliform test by the multiple-tube fermentationprocedure as a Most Probable Number (MPN) index. This is an index of the number of coliform bacteria that, more probably than any other number, would give the results shown bythe laboratory examination; it is not an actual enumeration. By contrast,direct plating methodssuch as the membrane filter procedure permit a direct count of coliform colonies. In bothprocedures coliform density is reported conventionally as the MPN or membrane filter countper 100 mL. Use of either procedure permits appraising the sanitary quality of water and the













Los métodos de análisis microbiológicos para la validación del programade
saneamiento empleados fueron:
Recuento de coliformes totales y escherichia coli: Método de la AOAC 991.14 (18ª Ed.).
Recuento de Staphylococcus aureus: Método de la AOAC 2003.07 (18ª Ed.).
Recuento de mohos y levaduras: Método de la AOAC 997.02 (18ª Ed.).
Recuento de microorganismos mesófilos aerobios viables: Método de la AOAC 990.12
(18ª Ed.).
Los planes de muestreo para laverificación microbiológica del panetón se diseñaron de
acuerdo con los criterios microbiológicos de calidad sanitaria e inocuidad para los
alimentos y bebidas de consumo humano (RM 615-2003-SA/DM).
Los métodos de análisis microbiológicos para la verificación microbiológica del
panetón fueron: Recuento de coliformes totales y escherichia coli: Método de la AOAC
991.14 (18ª Ed.)....