Articulos Sobre Tuberculosis

BMC Veterinary Research

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Molecular characterization of Mycobacterium bovis isolates from Ethiopian cattle
BMC Veterinary Research 2010, 6:28 doi:10.1186/1746-6148-6-28

Demelash Biffa (demelash_b@yahoo.com) Eystein Skjerve(eystein.skjerve@veths.no) James Oloya (joloya@uga.edu) Asseged Bogale (abogale@tuskegee.edu) Fekadu Abebe (fekadu.abebe@medisin.uio.no) Ulf Dahle (Ulf.dahle@fhi.no) Jon Bohlin (jon.bohlin@veths.no) Berit Djonne (berit.djonne@vetinst.no)

ISSN Article type Submission date Acceptance date Publication date Article URL

1746-6148 Research article 29 June 2009 27 May 2010 27 May 2010http://www.biomedcentral.com/1746-6148/6/28

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Molecularcharacterization of Mycobacterium bovis isolates from Ethiopian cattle
Demelash Biffa1†, Eystein Skjerve1, James Oloya2, Asseged Bogale3, Fekadu Abebe4, Ulf Dahle5, Jon Bohlin1, Berit Djønne6
1

Center for Epidemiology and Biostatistics, Norwegian School of Veterinary Science, P.O. Box

8146, N-0033, Oslo, Norway
2

Department of Veterinary Public Health and Preventive Medicine, MakerereUniversity P.O.

Box, 7062, Kampala, Uganda
3

College of Veterinary Medicine, Nursing and Allied Health Sciences, Tuskegee University,

Williams-Bowie, Bldg 114, Tuskegee, AL 36088,USA
4

Institute of General Practice and Community Medicine, Faculty of Medicine, University of Oslo, P.O. Box 1130 Blindern, Oslo, Norway

5

Department of Bacteriology and Immunology, Division of InfectiousDisease Control, Norwegian Institute of Public Health, P.O. Box 4404 Nydalen N0-0403 Oslo, Norway

6

Department of Animal Health, National Veterinary Institute, P.O.Box 750 dep, N-0106 Oslo, Norway
†

corresponding author

Email address: DB: ES: JO: AB: demelash_b@yahoo.com eystein.skjerve@veths.no joloya@uga.edu

abogale@tuskegee.edu fekadu.abebe@medisin.uio.no ulf.dahle@fhi.nojon.bohlin@veths.no berit.djonne@vetinst.no

FA: UD: JB: BD:

1

Abstract
Background: Bovine Tuberculosis (BTB) is a widespread and endemic disease of cattle in Ethiopia. Information relating to genotypic characteristics of Mycobacterium bovis strains affecting the cattle population in Ethiopia is limited. We carried out molecular characterization of M. bovis strains isolated from BTB infectedcattle using the spoligotyping technique. The relationship between distribution of spoligotypes and recorded variables was also investigated. A new approach that can numerically reflect the degree of genetic polymorphism in a M. bovis population was also developed. The study was conducted from July 2006 to January 2007 in cattle slaughtered at five representative abattoirs in Ethiopia. Results:Forty-five M. bovis isolates were obtained from 406 pathologic tissue specimens collected from 337 carcasses with lesions compatible with BTB. Twelve spoligotypes were identified from 34 distinct strains; with SB1176 as a dominant spoligotype (41.2 % of the isolates) followed by SB0133 (14.7 %). Comparison of spoligotypes with an M. bovis global database (http://www.mbovis.org) revealed six new...