Biologia

Abstract

Adult neurogenesis is a phenomenon that is extremely susceptible to be modified. It has been shown in animal models that manipulations such as cerebral ischemia, the specific injury ofbrain areas, altered neurogenesis. It has been shown to induce brain damage motor norepinephrine decreased pontine level that is related to motor deficits. The study discusses the relationship thatexists between neurogenesis and levels of norepinephrine in the brain damage motor. Wistar rats were used for experiments, divided into four groups, control group, group with a noradrenergic precursor(L-DOPS), a group with no precursor lesions noradrenergic motor and motor-injured group with noradrenergic precursor. Brain damage is induced by intracortical injection of FeCl2 (50 mM). The motordeficits will be assessed by the technique of the beam in the four groups. The animals received a daily intraperitoneal injection of BrdU (50 mg / kg) from the day of surgery, injury and so on to day 3 or20. Also used the technique of immunohistochemistry to observe the labeled neurons with BrdU using confocal microscopy were counted and the number of new neurons in the dentate gyrus (DG).Determination of norepinephrine levels will be made by high performance liquid chromatography with electrochemical detection decision (HPLC-ED). In addition, norepinephrine levels were analyzed by ANOVA forindependent groups with a post-hoc test of Tukey. In all cases the significance level was set to P ≤ 0.05. This study is expected to restore norepinephrine levels after pontine brain damage is related tomotor functional recovery and increases neurogenesis after brain damage, then the noradrenergic activity and neurogenesis are related processes necessary to achieve recovery functional.

ResumenLa neurogénesis adulta es un fenómeno que es extremadamente susceptible de ser modificado. Se ha mostrados en modelos animales que manipulaciones como la isquemia cerebral, la lesión específica...