Condroitin Sulfato: Determinación Fotométrica

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Method description
Titration Application Note T–83
Version 1
Sample
Chondroitin sulfate sodium
Sample preparationNo sample preparation required
Configuration
907 Titrando 1 x 2.907.0010
800 Dosino 4 x 2.800.0010
Dosing unit 5 mL 1 x 6.3032.150
Dosing unit 10 mL 1 x 6.3032.210
Dosing unit 50 mL 1 x6.3032.250
802 Rod Stirrer 1 x 2.802.0020
815 Robotic USB SP 1 x 2.815.0110
Sample beaker
250 mL
1 x 6.1432.320
Optrode (660 nm) 1 x 6.1115.000
Ecotrode plus 1 x 6.0262.100Electrode cable 1 x 6.2104.600
Solutions
Titrant
1-Hexadecylpyridi-nium chloride (also
called cetylpyridinium
chloride, CPC)
CAS 123-03-5
Dissolve approx. 280 mg
CPC in dist. water and makeup to 250 mL with dist.
water.
Chondroitin-4-sulfate
sodium salt
CAS 39455-18-0
Monobasic potassium
phosphate
CAS 7778-77-0
Dibasic potassium
phosphate
CAS 7758-11-4
Polysorbat80 CAS 90055-65-6
Phosphoric acid CAS 7664-38-2
Potassium hydroxide CAS 1310-58-3
Diluent pH = 7 Dissolve approx. 297 mg
KH2PO4, 492 mg K
2HPO4
,
and 250 mg Polysorbat 80
in dist.water and make up
to 1 L. Adjust with
potassium hydroxide or
phosphoric acid to a pH of
7.0 ± 0.2.
Analysis
Add x mL of sample solution in the titration vessel, add
30 mL of dist. water and50 mL diluent solution. Titrate
with CPC in MET mode using the Optrode (660 nm).
Parameters
Titration mode MET
Measurement drift 50 mV/min
Min. waiting time 0 s
Max. waiting time 26 s...