Biotecnologia

Tip: 1. To search the note, press CTRL-F keys in combination 2. To navigate the note, use the links.

We want to hear from you! Click here

DMEM Dulbecco’s Modified Eagle Media
PRODUCT DESCRIPTION A basal medium consisting of vitamins, amino acids, salts, glucose and a pH indicator. It contains no proteins or growth promoting agents. CATALOG NUMBERS BRAND LABELING INTENDED USE STABILITYSTORAGE CONDITIONS SHIPPING CONDITIONS QC SPECIFICATION PROTOCOLS AND APPLICATION NOTES FORMULATION PRODUCT DOCUMENTATION FAQ REFERENCES CATALOG NUMBERS Invitrogen offers several versions of DMEM including powder or liquid, with or without L-glutamine, High or Low Glucose, etc. The comparison table is a quick reference to help identify the catalog number that best suites your requirements: BaseCatalog Number 11965 11995 12430 21063 11971 12100 12800 11960 10313 31053 21013 21068 10569 10564 10566 11885 11966 12320 31600 11054 10567 Glucose level 4.5 g/L 4.5 g/L 4.5 g/L 4.5 g/L 4.5 g/L 4.5 g/L 4.5 g/L 4.5 g/L 4.5 g/L 4.5 g/L 4.5 g/L 4.5 g/L 4.5 g/L 4.5 g/L 4.5 g/L 1.0 g/L 0.0 g/L 1.0 1.0 1.0 1.0 Liquid or Powder Liquid Liquid Liquid Liquid Liquid Powder Powder Liquid Liquid Liquid LiquidLiquid Liquid Liquid Liquid Liquid Liquid Liquid Powder Liquid Liquid With 4mM Glutamine ● ● ● ● ● ● ● With GlutaMAX With 1mM Sodium Pyruvate ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● With 25mM HEPES Without Phenol Red Without Glucose Without Phosphate Without Methionine and Cystine Without Calcium

1

BRAND GIBCO® LABELING INTENDED USE Refer to specific product in our on-line catalog.Most versions have an intended use of in vitro diagnostic but some are intended for research only. STABILITY (back to top) Liquid: 12 months from date of production. Powder: 36 months from date of production. STORAGE CONDITIONS (back to top) This product should be stored at 2-8ºC, in the dark. SHIPPING CONDITIONS (back to top) This product is shipped at room temperature. QC SPECIFICATIONS (backto top) Test Sterility (Only for liquid) pH (only for liquid) pH before additions (only for powder) Osmolality Endotoxin Sp2 Toxicity Assay Specification Negative 7.0-7.4 for most versions. May vary depending on specific product. 5.9-6.3 for most versions. May vary depending on specific product. Will vary depending on specific product Warning limit when >0.5 EU/ml 80-200% of control

PROTOCOL ANDAPPLICATION NOTES (back to top) General Information: DMEM is a basal medium consisting of vitamins, amino acids, salts, glucose and a pH indicator. It contains no proteins or growth promoting agents. Therefore, it requires supplementation to be a “complete” medium. It is most commonly supplemented with 510% Fetal Bovine Serum (FBS). DMEM utilizes a sodium bicarbonate buffer system (3.7 g/L) andtherefore requires artificial levels of CO to maintain the required pH. 7-10% CO2 is optimal but many researchers successfully use it in 5% CO2. The potential problem with too low CO2 level is that the pH may become too high. When exposed to ambient levels of CO2, the sodium bicarbonate in the medium will cause DMEM to become basic very rapidly. This why one will often observe a half used bottle ofmedia appears purple, indicating a rise in pH. Powder media is formulated without sodium bicarbonate because it tends to gas off in the powdered state. Powder media requires the addition of 3.7 g/L of sodium bicarbonate upon dissolving it in water. Historical information: In 1955 Harry Eagle discovered that in addition to the ten essential amino acids (arginine, histidine, isoleucine, leucine,lysine, methionine, phenylalanine, threonine, tryptophan and valine), mammalian cells also required cystine and tyrosine and glutamine. His original formulation, known as Basal Media Eagle’s (BME), also contained eight B vitamins. The BME as originally developed was for HeLa cells and mouse fibroblasts although it was later extended to human diploid lines e.g. WI-38 and MRC-5. There are a number...