Estudio Origen Sexualidad De Simon Levay (Inglés)

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A Difference in Hypothalamic Structure between Heterosexual and Homosexual Men Author(s): Simon LeVay Source: Science, New Series, Vol. 253, No. 5023 (Aug. 30, 1991), pp. 1034-1037 Published by: American Association for the Advancement of Science Stable URL: http://www.jstor.org/stable/2878803 Accessed: 03/08/2010 17:04
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http://www.jstor.org growth, nudear segregation,DNA repair,and meiosis, and deletion of HRR25 resultsin cell cycle defects.These phenotypes, coupled with the similarityof the HRR25 sequence to the sequenceof the Rafc-Mos protein kinasesubgroup (Fig. 3A), suggest that HRR25 might play a similarrole in S. cerevisiae growth and development. The defects in DNA doublestrandbreakrepairand aberrant growth propertiesrevealed by mutations in the HRR25 kinaseextend the possible functions of protein kinasesin cell growth and place HRR25 with CDC7 in a functionalcategoryof yeast kinase associatedwith DNA metabolism.
REFERENCES AND NOTES 1. P. C. Hanawalt, P. K. Cooper, A. K. Ganesan, C. A. Smith, Annu. Rev. Biochem. 48, 783 (1979); L. Thompson, in Genetic Recombination, KucherlaR. pati and G. R. Smith, Eds. (AmericanSociety for Microbiology, Washington, DC, 1989), pp. 597631; E. C. Friedberg, Microbiol.Rev. 52, 70 (1988). 2. L. Hartwell and T. W. Weinert, Science 246, 629 (1990); T. W. Weinert and L. Hartwell, ibid. 241, 317 (1988); R. Schliestl, P. Reynolds, S. Prakash, L. Prakash, Mol. Cell. Biol. 9, 1882 (1989). 3. R. Haynes and B. A. Kunz, in MolecularBiology of the Yeast Saccharomyces, Strathern, E.Jones, J. J. Broach, Eds. (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1981), pp. 371-414; J. Game, in Yeast Genetics: Fundamentaland Applied Aspects, J. F. T. Spencer, D. M. Spencer, A. R. W. Smith, Eds. (Springer-Verlag, New York, 1983), pp. 109-137. 4. R. Kostriken and F. Hefron, Cold Spring Harbor Symp. Quant. Biol. 49, 89 (1984); J. Nickoloff, J. D. Singer, M. F. Hoekstra, F. Hefron,J. Mol. Biol. 207, 527 (1989). 5. R. E. Malone and R. E. Esposito, Proc. Natl. Acad. Sci. U.S.A. 77, 503 (1980); B. Weiffenbach and J. Haber, Mol. Cell. Biol. 1, 522 (1981). 6. D. Schild et al., Curr. Genet. 7, 85 (1983); G. Cole et al., Mol. Cell. Biol. 9, 3101 (1989). 7. Saccharomyces cerevisiae strain K264-5B (26) (MATa ho ura3 cantRtyrl his7 lys2 ade5 metl3 trp5 leul ade52) was used for themutant isolation. K2645B was transformedwith a URA3-based integrating plasmid that contains a GALl-regulated HO endonuclease (4), and a transformantwas mutagenized to approximately 50% survival with ethyl methanesulfonate. The culture was spread onto rich medium containing glycerol (to avoid formation of petites), colonies were allowed to form at 30?C, and plates were replicated to glucose...
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