Produccion De Lipasa Por Pseudonomas

American-Eurasian J. Agric. & Environ. Sci., 5 (4): 556-563, 2009
ISSN 1818-6769
© IDOSI Publications, 2009

Degradation of Castor Oil and Lipase Production by Pseudomonas aeruginosa
1

Amro A. Amara and 2Soheir R. Salem
1

Protein Research Department,
Genetic Engineering and Biotechnology Research Institute,
Mubarak City for Scientific Research and Technology Applications,Alexandria, Egypt
2
Biological and Geological Science Department, Faculty of Education,
Alexandria University, Alexandria, Egypt
Abstract: Many Enzymes produced by Pseudomonas aeruginosa have been used in commercial scales, lipase
is an example. P. aeruginosa has been investigated intensively during past decays regarding its medical and
industrial importance. It will establish in different wastestreatment processes such as oil waste bioremediation.
In this study, two strains of P. aeruginosa were used to produce lipase from Castor oil aiming to control the
different castor oil wastes and technical lipase production. Both strains are able to grow on media consists of
20 g/l Castor oil and 3 g/l Yeast extract at 37°C. The lipase enzyme activity at 37, 60 and 75°C in presence of 0.4,
0.8, 2and 2.8 mg/ml p-nitrophenyl palmitate were investigated. The Vmax and Km using of both Hanes and
Lineweaver-Burk plots were determined. Lipase produced from the two strains revealed mesophilic and
thermophilic activities. The maximum activity at 37°C was 1121.00 and 470.82 Units/ml, while at 75°C was 358.72
and 147.18 Unit/ml for strain 1 and strain 2, respectively. We recommends for using P.aeruginosa lipases for
castor oil waste control and for the production of mesophilic and thermophilic lipases under mesophilic
conditions.
Key words: Castor oil % Pseudomonas aeruginosa % Kinetic % Hanes plot % Lineweaver-Burk
INTRODUCTION

one of the most technical enzymes which represent 1
billion USD in 1999 [10]. Part of these enzymes was the
thermostable enzymes which were bettersuited for
harsh industrial processes and constitute more tha 65%
of the global market [10-12]. Enzymes have many
applications especially in paper industry, detergent,
drugs, degradation of different wastes, textile, food,
pharmaceutical, leather, degumming of silk goods,
manufacture of liquid glue, cosmetics, meat tenderization,
cheese production, growth promoters etc [10,13].
Thermostableenzymes can be produced by both of
thermophilic and mesophilic microbes [14]. Some reports
described Psycrophiles as a source of some stable
enzymes but using thermophils reduce the risk of
contamination by common mesophiles. Pseudomonads a
general name referred to a part of a large, heterogeneous
and ubiquitous (Being present everywhere at once) group
of microorganisms [15,16]. The biggenetic material of the
P. aeruginosa support the idea of using this microbes in
biotechnological applications. The diversity of P.
aerginosa different strains and that each strain should be

The castor oil from Ricinus communis represent a
potential bioproduct and used in many technical and
medical applications. During castor oil extraction process
and refining as well as its use in differentindustrial
applications like bio-diesel production, different types of
wastes elevated. Using different lipases for castor oil
wastes control has been described by authors such as
lipases from ground oats, castor bean itself, Aspergillus
oryzae, Pseudomonas aeruginosa KKA-5, Pseudomonas
fluorescens NS2W, A. oryzae, Geotrichum candidum,
Candida rugosa, Pseudomonas cepacia [1-7].
Differentstudies describe an efficient low-cost
method for biodetoxification of castor oil has been
reported. As an example, an efficient method for crude
caster oil bioremediation was described by Godoya et al
[8]. Lipases have been used for degrading castor oil either
for production of new products or for technical lipases
production. A method for bioconversion of castor oil to
ricinoleic acid was...