Bergeys Id Chart

Identification flow charts

Bergey’s Manual of Determinative Bacteriology
All of the unknowns will fall into the following groups in Bergey's Manual of Determinative Bacteriology (The pink book on the shelf in the laboratory).

GROUP 4
Description: Gram Negative, Aerobic/Microaerophilic rods and cocci Key differences are: pigments/fluorescent, motility, growth requirements, denitrification,morphology, and oxidase, read Genera descriptions Examples: Acinetobacter, Pseudomonas, Beijerinckia, Acetobacter

GROUP 5
Description: Facultatively Anaerobic Gram negative rods Key differences are: growth factors, morph., gram rxn., oxidase rxn., read Genera descriptions Examples: Family Enterobacteriaceae and Vibrionaceae

GROUP 17
Description: Gram-Positive Cocci Key differences are:oxygen requirements, morph., growth requirements (45°C and supplements), read Genera descriptions Examples: Micrococcus, Staphylococcus, Streptococcus, Enterococcus, Lactococcus, Aerococcus

GROUP 18
Description: Endospore-Forming Gram positive rods and cocci Key differences are: oxygen requirements, motility, morph, catalase Examples: Bacillus, Clostridium

GROUP 19
Description: Regular,Nonsporlating Gram positive rods Key differences are: morph., oxygen require, catalase Examples: Lactobacillus, Listeria

GROUP 20
Description: Irregular, Nonsporlating Gram-positive rods Key differences are: catalase, motility, morph., read Genera descriptions Examples: Actinomyces, Corynebacterium, Arthrobacter, Propionibacterium

GROUP 21
Description: Weakly Gram-Positive Nonsporlating AcidFast Slender Rods Key differences are: acid fast, growth Examples: Mycobacterium

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Identification flow charts

Differentiation via Gram stains and cell morphology.

Gram Stain & Morphological Flowchart
Some Examples

Gram Positive
Cocci Bacilli
Filamentous Round in clusters & tetrads:
Staphylococcus Micrococcus Peptococcus

Oval shape in Chains:

StreptococcusPeptostreptococcus Enterococcus

Club-shaped and/or in palisades:

Corynebacterium Listeria Erysipelothrix Mycobacterium (Acid Fast) Propionobacterium

Spore-bearing, large, uniform:
Bacillus Clostridium

Extensive branching:

Actinomyces Arachnia Nocardia (partially Streptomyces
acid-fast)

Branching rudimentry or absent:
Erysipelothrix Lactobacillus Eubacterium

Gram Negative
Cocci BacilliNeisseria Veillonella

Medium-sized cocco-bacillary:
Acinetobacter Moraxella

Tiny-coccobacillary:
Brucella Bordetella Bacteroides

Pleomorphic coccobacillary & filamentous:

Exaggerated pointed ends:

Haemophilus Bacteroides Pasteurella Francisella Actinobacillus Eikenella Cardiobacterium Flavobacterium

Fusobacterium nucleatum

Curved, comma-shaped:
Vibrio CampylobacterSpirillium

Uniformly Bacillary:

Coiled & sphero-plastic:
Streptobacillus Fusobacterium

Enterobacteriaceae Pseudomonas Aeromonas Alcaligenes Chromobacterium

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Identification flow charts

Gram Positive Rods ID Flowchart

Gram Positive Rods
Bacillus spp. Clostrididium spp. Corynebacterium spp. Lactobacillus spp. Mycobacterium spp.

Mycobacterium smegmatis Spore Forming + Bacillus spp.Clostridium spp Corynebacterium spp. Lactobacillus spp. Mycobacterium spp. Acid Fast +

Strict Anaerobes +
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Corynebacterium spp Lactobacillus spp. -

Clostridium spp.

See separate flowchart.

Bacillus spp.

Catalase + Corynebacterium spp. Lactobacillus spp.

Starch Hydrolysis Glucose Ferm. Activity + Corynebacterium kutsceri Corynebacteriumxerosis
Acid & Gas Acid

Lactobacillus fermenti

Lactobacillus casei Lactobacillus delbrueckii

Mannitol + Lactobacillus casei Lactobacillus delbrueckii

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Identification flow charts

Bacillus spp. ID Flowchart

Bacillus spp.
B. subtilis B. cereus B. megaterium B. stearothermophilus B. polymyxa B. mycoides B. macerans B. thuringiensis B. macquariensis B. licheniformis B. lentus...