Chlamydia

Chlamydia Trachomatis IgG Rev.1RC

Antibody Index Interpretation 1.1 Detectable antibody to C. trachomatis IgG by ELISA LIMITATIONS OF THE TEST 1. The test results obtained using this kit serve only as an aid to diagnosis and should be interpreted in relation to the patient’s history, physical findings and other diagnostic procedures. 2. Lipemic or hemolyzed samples may cause erroneous results.REFERENCES 1. Poussin M; Fuentes V; Corbel C; Prin L; Eb F; Orfila J. Capture-ELISA: a new assay for the detection of immunoglobulin M isotype antibodies using Chlamydia trachomatis antigen. J Immunol Methods, 1997; 204(1):1-12. 2. Dereli D; Coker M; Ertem E; Serter D; Tana¸c R; Tez E. Chlamydial infection in infants. J Trop Pediatr 1996; 42(4):233-6. 3. Bas S; Cunningham T; Kvien TK; Glenn as A;Melby K; Vischer TL. The value of isotype determination of serum antibodies against Chlamydia for the diagnosis of Chlamydia reactive arthritis. Br J Rheumatol 1996; 35(6):542-7 4. Gencay M; Koskiniemi M; Saikku P; Puolakkainen M; Raivio K; Koskela P; Vaheri A. Chlamydia trachomatis seropositivity during pregnancy is associated with prenatal complications Clin Infect Dis 1996; 21(2):424-6. 5.Verkooyen RP; Van Lent NA; Mousavi Joulandan SA; Snijder RJ; van den Bosch JM; Van Helden HP; Verbrugh HA. Diagnosis of Chlamydia pneumoniae infection in patients with chronic obstructive pulmonary disease by micro-immunofluorescence and ELISA. J Med Microbiol 1997; 46(11):959-64 2008-12-18

Chlamydia Trachomatis IgG ELISA
Catalog No.: CT054G (96 Tests) INTENDED USE The Calbiotech ChlamydiaTrachomatis IgG Kit is intended for the detection of IgG antibody to Chlamydia Trachomatis in human serum or plasma. SUMMARY AND EXPLANATION C. trachomatis is an intracellular parasite pathogen that is similar in cell wall structure to gramnegative bacteria. It is the most common sexually transmitted disease (STD) in the US with more than 4 million cases reported annually. The main sites of infectioninclude the GU tract and rectum but conjunctivitis, perihepatitis and reactive arthritis may result. The infection is often asymptomatic, making it difficult to diagnose; as many as 2/3 of infected women are asymptomatic. Women develop mucopurulent cervicitis, and irregular menstrual bleeding or abdominal pain may occur in about 40% of these women. PID is found in about 5% of women. The infection isusually symptomatic in men with dysuria and white/clear discharge occurring. Epididymitis is common. The infection incubates in 7 to 21 days and is commonly found with a second STD pathogen. IgG and IgM antibodies to C. trachomatis can be detected with 2-4 weeks after exposure. IgG remains positive, but the antibody level can drop overtime. ELISA can detect C. trachomatis IgM antibody for manymonths after infection. PRINCIPLE OF THE TEST Diluted patient serum is added to wells coated with purified antigen. IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow thehydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample. MATERIALS PROVIDED Microwells coated with C. trachomatis antigen Sample Diluent: 1 bottle (ready to use) Calibrator: 1 Vial (ready to use) Positive Control: 1 vial (ready to use) Negative Control: 1 vial (ready to use) Enzyme conjugate: 1 bottle (ready touse) TMB Substrate: 1 bottle (ready to use) Stop Solution: 1 bottle (ready to use) Wash concentrate 20X: 1 bottle 96 Tests 12x8x1 22 ml 1.5ml 1.5ml 1.5ml 12ml 12ml 12ml 25ml

For Research Use Only. Not for use in Diagnostic Procedures.

Cat#: CT054G (96 Tests) For Order and Inquiries, please contact Calbiotech Inc., 10461 Austin Dr, Spring Valley, CA, 91978 Tel (619) 660-6162, Fax (619)...