Genetica Del Virus Sv40

GRAFICO

TEXTO (PROPIEDADES Y CARACTERISTICAS)

Tarea SV40
General Description
DNA Plasmid 'Tarea SV40'
Tarea SV40
Local object
Created: 03/11/11 04:47p.m.
Last Modified: 03/11/11 04:47p.m.
length: 10617 bp
storage type: Designed
form: Circular
Standard FieldsKeywords: Viktor
Comments
Annotations
Component Fragments
#1: FRAGMENT of <a href="vnti:dnarna/BPV1?sel=7631-7630">BPV1</a>
parent position: from 7631 to 7630
original length: 7945
molecule position: from 10303 to 7630
Left Terminus
ApaLI site #2
Right Terminus
ApaLIsite #2
#2: LINKER of ApaLI (right part)
molecule position: from 7631 to 7639
#3: FRAGMENT of <a href="vnti:dnarna/SV40?sel=2538-5191">SV40</a> (Complementary)
parent position: from 2538 (+ fill of 5191) to 4
original length: 2658
molecule position: from 7640 to 10297
Left Terminus
BamHI site #1Completely filled in
Right Terminus
AvrII site #2
Completely filled in
#4: LINKER of ApaLI (left part)
molecule position: from 10298 to 10302
Design Description
Step #1
Result Molecule: Tarea SV40

Recipient: BPV1
Left Terminus: ApaLI #2 (total 2)Right Terminus: ApaLI #2 (total 2)
Partial digestion: 1 sites inside required fragment, 2 sites total

Donor: SV40 (Complementary)
Left Terminus: BamHI #1 (total 1)
Completely filled in
Modified by linker ApaLI
Flank Region: 153
Right Terminus: AvrII #2 (total 2)
Completelyfilled in
Modified by linker ApaLI
Flank Region: 28
Full digestion

Ligation: cohesive termini at both junctions

Preselection: dephosphorylation

Transformation system: Bacteria
Extra-chromosomal replication

Recommended method(s) for clone analysis:Restriction Analysis

Recommended enzyme(s) for restriction analysis:
AgeI

Recommended enzyme(s) for restriction analysis
defining clone with required fragment orientation:
AvrII
ApaLI

Recommended oligonucleotide for colony hybridization
of cloned fragment inresulting molecule:
CTAGGCTTTTGCAAAAAGCT

Recommended primers for PCR amplification
of cloned fragment in resulting molecule:
Sense Primer:
ATGTCTGTACCGCCATCGGTGCAC
Antisense Primer:
ACCTATATCGGTGCACGGGG

Important Restriction Sites:
Cloned fragment may be cut by AvrII andApaLI
Recombinant lacks sites of:
AfeI ApaI AscI AsiSI BsiWI BssHII
EcoICRI EcoRV FseI M.ApaI M.BstVI M.EcoRV
M.NgoMIV M.Rrh4273I M.SacI M.SnaBI MscI NaeI
NgoMIV NotI PacI PmeI PspOMI PvuI
SacI SacII SalI SciI SnaBI SrfI
Sse232I SwaI XhoI
Unique sites outside cloned fragment:
AatII Acc65I AclIAflII BspEI BstBI EagI EcoRI
FspI KpnI M.AclI M.Cfr9I M.EcoRI M.KpnI M.PhiBssHII M.SsoI
M.XbaI M.XmaIII MluI NruI SmaI UthSI XbaI XmaI
ZraI
Feature Map
CDS (12 total)
E6
Start: 49 End: 501
transforming protein
E7
Start: 449 End: 859
cop gene
E1...