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Applied Surface Science 255 (2008) 1584–1587

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Microscopy and chemical imaging of Behcet brain tissue
Monika Aranyosiova a,b,1,*, Miroslav Michalka a,1, Martin Kopani c,3, Boris Rychly d,4, Jan Jakubovsky c,3, Dusan Velic a,b,1,2

International Laser Center, Ilkovicova3, SK-84104 Bratislava, Slovakia Department of Physical and Theoretical Chemistry, Faculty of Natural Sciences, Comenius University, Mlynska dolina, SK-84215 Bratislava, Slovakia c Institute of Pathology, Faculty of Medicine, Comenius University, Sasinkova 4, SK-81108 Bratislava, Slovakia d Institute of Pathology, Derer’s Hospital, Limbova 5, SK-83305 Bratislava, Slovakia



Article history: Available online 14 May 2008 Keywords: TOF-SIMS EDX SEM Behcet brain tissue Chemical imaging

Chemical composition and distribution of molecules and elements in a human brain tissue of Behcet diseased patient are of interest. Behcet disease is a multi-system disorder of which pathogenesis and chemical causality are still uncertain. Time-of-flightsecondary ion mass spectrometry is used along with scanning electron microscopy and energy dispersive X-ray analysis providing complex composition in Behcet disease and control tissues. Determined organic compounds are represented by fragments of carbohydrates, phospholipids, amino acids, and peptides. The distributions of inorganic species are well represented by heavy trace elements and by oxides inpositive and negative polarities of time-of-flight secondary ion mass spectrometry, respectively. Organic and inorganic compounds are qualitatively determined in both samples, Behcet and control, providing complementary chemical images. The complementary chemical images interestingly change with the quantitative regression of organic compounds distribution, characteristic for the healthy control,towards inorganic compounds distribution, characteristic for Behcet tissue. ß 2008 Elsevier B.V. All rights reserved.

1. Introduction Behcet disease (BD) is a rare multi-system disorder of which pathogenesis and chemical causality are still uncertain. Chemical characterization of BD tissue providing detailed elemental and molecular compositions might be useful for an investigation of the possiblecausality. BD was mostly characterized from its medical aspects with respect to the oxidative processes along with the presence of trace elements [1–6]. Although pathological influence of trace heavy elements was proposed [7], a direct comparison between the organic and inorganic compounds distributions is novel. Contribution of this paper is based on time-of-flight secondary ion mass spectrometry(TOF-SIMS) and on energy dispersive X-ray analysis (EDX) analysis providing the complex chemical composition of BD brain tissue along with images from

scanning electron microscopy (SEM) and the chemical imaging capabilities of TOF-SIMS. Among the methods determining the chemical composition in biological samples, TOF-SIMS technique has certain advantages with an extra scanning feature to localizedifferent chemical species on the sample surface providing twodimensional chemical images. The aim of this study was to differentiate between the control and BD tissues using TOF-SIMS and EDX/SEM techniques and to provide a complete chemical characterization in the form of images. 2. Experimental For physical and chemical examinations, necrotic material was obtained from the pons cerebrum of a 39years old man suffering from neurological form of morbus BD. The control sample of this investigation was obtained from a postmortem patient of the same age clearly without any neurological disease. Note that the measurements were performed on both samples at least on three different areas providing necessary basic statistics. The only one case study of neurological BD patient was dictated by...
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