CELL TISSUE TAXONOMY
Objectives: After completing this lab the student will:
1. Understand what cellular respiration, its importance and the mainsteps of it.
2. Differentiate between aerobic respiration and anaerobic.
3. Differentiation of lactic and alcoholic fermentation, and learn their applications.
4. Understand how fermentation occursfrom various carbohydrates.
5. Measure the rate of aerobic respiration in various organisms.
6. Knowing how to carry out a titration.
Cells carry out various processes to maintain normaloperation, many of which require energy. Cellular respiration is a series of reactions by which the cell degrades organic molecules and produces energy. All living cells carrying out cellular respiration toobtain energy for their functions. Is usually used glucose as raw material, which is metabolized to carbon dioxide and water, producing energy that is stored as ATP (adenosine triphosphate).
C6H12O6H2O + CO2 + Energy (ATP)
Ó Ó respiration NCELULARANAER BICA
A. Alcoholic fermentation in yeast In this exercise we will study the alcoholic fermentation process carried out by yeasts.
Theseorganisms perform aerobic respiration in the presence of oxygen and anaerobic respiration in absence. The yeast used is Saccharomyces cerevisiae, the same used for the production of bread, beer and wine. Inthe alcoholic fermentation produces carbon dioxide and ethyl alcohol (ethanol). The carbon dioxide creates the fizz in beer and makes the bread "rise" in the oven. The ethanol produced is alcohol inbeers and wines. Various solutions will be used to determine which carbohydrates can be metabolized by fermentation.
M A T E R I A L E S
• Rack for test tubes
• Four test tubes medium
• Four1-ml pipettes
• Four disposable Pasteur pipettes
• wax pencil
• An envelope of yeast
• Sugar or molasses
• vessel (beaker) of 200 ml
• Role of paraffin (Parafilm)
• Solution of sucrose,...