Yuca

MASS PRODUCTION OF FUNGAL PATHOGENES FOR INSECT CONTROL
INSECT PATHOLOGY MANUAL

Section

VII
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CONTENTS
I N T R O D U C T I O N ............................................................................................................................. 5 1 . G E N E R A L P R O C E D U R E S FOR TWO STAGE PRODUCTION OF FUNGAL PATHOGENS ....... 7 Culturemaintenance..............................................................................................................................................................................................7 Liquid media...........................................................................................................................................................................................................7 Solid substrate.........................................................................................................................................................................................................7 QualityControl.......................................................................................................................................................................................................8 2 . C U L T U R E M A I N T E N A N C E .......................................................................................................... 9 Organising a culturecollection............................................................................................................................................................................9
Storage of isolates.................................................................................................................................................................................................................................. 10 Specialised methods............................................................................................................................................................................................................................... 10

Stage 1:................................................................................................................................................................................................................................................... 7 Stage2:................................................................................................................................................................................................................................................... 7 Drying andExtraction........................................................................................................................................................................................................................... 8

3 . M A S S P R O D U C T I O N U S ING THE TWO STAGE ....................................................................... 1 1 Technique Preparation and inoculation of brewers' yeast/sucrose (by/suc) broth...............................................................................11
Preparation of BY/SUC broth............................................................................................................................................................................................................ 11 Stage1.................................................................................................................................................................................................................................................. 11 Inoculation of BY/SUC broth............................................................................................................................................................................................................. 11 Preparation and inoculation of rice substrate...