Rami

Rami saadat
Mr. jayax
Science
2/3/13
Chromatography lab

Aim: which of the dyes will expand more?
Theory
Chromatography: is a technique that is used to separate and to identify components of a mixture. This analytical technique has a wide range of applications in the real world since many substances are mixtures of chemical compounds. The components to be separated are distributedbetween two phases: a stationary phase and a mobile phase. stationary phase is the solid called the filter paper and mobile phase is the liquid.

How Does It Work?
A mixture of various components enters a chromatography process, and the different components are flushed through the system at different rates. These differential rates of migration as the mixture moves over adsorptive materialsprovide separation. Repeated sorption/desorption acts that take place during the movement of the sample over the stationary bed determine the rates.
Why Is It So Special?
In any chemical or bioprocessing industry, the need to separate and purify a product from a complex mixture is a necessary and important step in the production line. Today, there exists a wide market of methods in which industriescan accomplish these goals. Chromatography is a very special separation process for a multitude of reasons! First of all, it can separate complex mixtures with great precision. Even very similar components, such as proteins that may only vary by a single amino acid, can be separated with chromatography. In fact, chromatography can purify basically any soluble or volatile substance if the rightadsorbent material, carrier fluid, and operating conditions are employed. Second, chromatography can be used to separate delicate products since the conditions under which it is performed are not typically severe. For these reasons, chromatography is quite well suited to a variety of uses in the field of biotechnology, such as separating mixtures of proteins.
Polarity:
Is the property orcharacteristic that produces unequal physical effects at different points in a body or system, as a magnet or storage battery. There are colors that have high level of polarity that combined with water that is polar that help when doing an experiment with chromatography and help the dyes to expand a lot.
sources:
http://www.biologyjunction.com/paper_chromatography_report.htmhttp://www.uwplatt.edu/chemep/chem/chemscape/labdocs/catofp/chromato/paper/paper.htm#stationary
http://wiki.answers.com/Q/What_are_stationary_and_mobile_phase_of_chromatography
http://cemca.org/andcollege/andcwebsite/subject01/CHEtext.htm
http://chemlab.truman.edu/CHEM100Labs/PAPER%20CHROMATOGRAPHY.pdf

Hypothesis
If I used different color marker then the yellow dying will expand more because yellow is polar thesame as water that was used as Mobil phase so they combine and move.

Variables
Independent:
water colored markers: purple, green, black, blue, orange, yellow, and red.
Dependent:
Ruler: iam going to use It to measure how much the dying will expand.

Control:
Problem | Solution |
Lost your pencil | Get new one |
Forget the markers | Borrow a new one |
Forget the ruler | borrowone |
Lost the chromatography paper | Ask your friend for one |
Don’t have the solution used in the experiment | Ask the teacher to give you some |
Don’t have enough time to finish the experiment | Do it at home. |

Materials
1) Water colored marker: purple, green, black, blue, orange, yellow, and red.
2) Chromatography paper
3) Ruler
4) Pencil
5) Adequate amount ofwater
6) Stopwatch
7) Notebook
8) Cups
9) Camera

Methods

1) Get chromatography paper, ruler, pencil, water marker, cups, and water.
2) Draw a line on the paper using the paper and pencil.
3) Then with different water color marker draw points on the line.
4) Then insert water in the cup
5) then Put the paper in the water
6) the paper in the solution...