Allogeneic Mesenchymal Stem Cells Restore Cardiac Function In Chronic Ischemic Cardiomyopathy Via Trilineage Differentiating Capacity
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Publicado: 2 de noviembre de 2012
Allogeneic mesenchymal stem cells restore cardiac function in chronic ischemic cardiomyopathy via trilineage differentiating capacity
falseQuevedo, Henry C; Hatzistergos, Konstantinos E; Oskouei,Behzad N
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; Feigenbaum, Gary S; Rodriguez, Jose E; et al..Proceedings of the National Academy of Sciences of the United States of America106. 33 (Aug 18, 2009):14022.
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The mechanism(s) underlying cardiac reparative effects of bone marrow-derived mesenchymal stem cells (MSC) remain highly controversial. Here we tested the hypothesisthat MSCs regenerate chronically infarcted myocardium through mechanisms comprising long-term engraftment and trilineage differentiation. Twelve weeks after myocardial infarction, female swine receivedcatheter-based transendocardial injections of either placebo (n = 4) or male allogeneic MSCs (200 million; n = 6). Animals underwent serial cardiac magnetic resonance imaging, and in vivo cell fatewas determined by co-localization of Y-chromosome (Ypos) cells with markers of cardiac, vascular muscle, and endothelial lineages. MSCs engrafted in infarct and border zones and differentiated intocardiomyocytes as ascertained by co-localization with GATA-4, Nkx2.5, and a-sarcomeric actin. In addition, Ypos MSCs exhibited vascular smooth muscle and endothelial cell differentiation, contributing tolarge and small vessel formation. Infarct size was reduced from 19.3 ± 1.7% to 13.9 ± 2.0% (P < 0.001), and ejection fraction (EF) increased from 35.0 ± 1.7% to 41.3 ± 2.7% (P < 0.05) in MSCbut not placebo pigs over 12 weeks. This was accompanied by increases in regional contractility and myocardial blood flow (MBF), particularly in the infarct border zone. Importantly, MSC engraftment...
falseQuevedo, Henry C; Hatzistergos, Konstantinos E; Oskouei,Behzad N
Presione la tecla Escape para cerrar
; Feigenbaum, Gary S; Rodriguez, Jose E; et al..Proceedings of the National Academy of Sciences of the United States of America106. 33 (Aug 18, 2009):14022.
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La traducción del texto completo puede demorar entre 40 y 60 segundos; los documentos de mayor tamañopueden demorar más.
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The mechanism(s) underlying cardiac reparative effects of bone marrow-derived mesenchymal stem cells (MSC) remain highly controversial. Here we tested the hypothesisthat MSCs regenerate chronically infarcted myocardium through mechanisms comprising long-term engraftment and trilineage differentiation. Twelve weeks after myocardial infarction, female swine receivedcatheter-based transendocardial injections of either placebo (n = 4) or male allogeneic MSCs (200 million; n = 6). Animals underwent serial cardiac magnetic resonance imaging, and in vivo cell fatewas determined by co-localization of Y-chromosome (Ypos) cells with markers of cardiac, vascular muscle, and endothelial lineages. MSCs engrafted in infarct and border zones and differentiated intocardiomyocytes as ascertained by co-localization with GATA-4, Nkx2.5, and a-sarcomeric actin. In addition, Ypos MSCs exhibited vascular smooth muscle and endothelial cell differentiation, contributing tolarge and small vessel formation. Infarct size was reduced from 19.3 ± 1.7% to 13.9 ± 2.0% (P < 0.001), and ejection fraction (EF) increased from 35.0 ± 1.7% to 41.3 ± 2.7% (P < 0.05) in MSCbut not placebo pigs over 12 weeks. This was accompanied by increases in regional contractility and myocardial blood flow (MBF), particularly in the infarct border zone. Importantly, MSC engraftment...
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