Biologo

In vitro development of hydrocarbonoclastic microorganisms Proteus sp.,
Hernández et al.
OPTIMAL PARAMETERS FOR In Vitro DEVELOPMENT
OF THE HYDROCARBONOCLASTIC MICROORGANISM
Proteus sp.
M.A.Hernández-Rivera1, M.E. Ojeda-Morales1, J.G. Martínez–Vázquez2*, V.M.
Villegas-Cornelio3 and Y. Córdova-Bautista1.
1División Académica de Ingeniería y Arquitectura, Universidad Juárez Autónoma deTabasco,
Km. 1 Carretera Cunduacán-Jalpa, Colonia Esmeralda, Cunduacán, Tabasco, México, CP: 86040.
2Centro de Apoyo Pedagógico, Universidad de Atacama, Av. Copayapu 485, Copiapó, Región de
Atacama,Chile, CP: 1530000. 3Dpto. de Ciencias Químico-Biológicas, Universidad Popular de la
Chontalpa, Km. 2 Carretera Cárdenas-Huimanguillo, Cárdenas, Tabasco, México. CP: 86500.
*Corresponding author:gabrielm81@hotmail.com
ABSTRACT
In México, there are extensive areas polluted by oil spills. Currently, bioremediation
technologies have been developed, using microorganisms to clean up oil sites. Inthis
study, we evaluated the development of a hydrocarbonoclastic bacterial strain, using a
completely randomized 3x3x4 factor arrangement: three temperatures, three pH, and
four nutrients. Wecollected samples of soil contaminated with 3.45 x 105 mg kg-1
(345,000 ppm) Total Petroleum Hydrocarbons (TPH), located at the Ejido José N.
Rovirosa, Huimanguillo, Tabasco, México. The samples weregrown in a culture
medium Nutrient Agar (NA), obtaining a bacterial strain, which was characterized and
classified as Proteus sp. The strain was grown in a combined carbon culture medium
and then in aliquid mineral medium with crude oil as sole carbon source. Analysis of
variance and mean test were performed, using the SPSS-11.0 statistical software. The
microorganisms showed the highestpopulation growth in the treatment
N2=Triple17pH1=4.0T1=29ºC with a value of 4.6 x 1010 CFU mL-1. To reach, by
bioaugmentation, the same development of Proteus sp in a conditioned soil would allow
us to...