Extraccion protwinas
Hydrophobic Interaction and Reversed Phase Chromatography
Principles and Methods
Handbooks from GE Healthcare
Antibody Purification
Handbook 18-1037-46
Microcarrier cell culture The Recombinant Protein Handbook
Protein Expression and Simple Purification 18-1142-75 Principles and Methods 18-1140-62
2-D Electrophoresis
using immobilized pH gradientsProtein Purification
Handbook 18-1132-29
Principles and Methods 80-6429-60
Ion Exchange Chromatography & Chromatofocusing
Principles and Methods 11-0004-21
Hydrophobic Interaction and Reversed Phase Chromatography
Principles and Methods 11-0012-69
Affinity Chromatography
Principles and Methods 18-1022-29
Percoll
Methodology and Applications 18-1115-69
Gel FiltrationPrinciples and Methods 18-1022-18
Ficoll-Paque Plus
For in vitro isolation of lymphocytes 18-1152-69
Expanded Bed Adsorption
Principles and Methods 18-1124-26
GST Gene Fusion System
Handbook 18-1157-58
Hydrophobic Interaction and Reversed Phase Chromatography
Principles and Methods
Handbook 11-0012-69 AA 1
Contents
Introduction......................................................................................................................................... 7
Symbols .................................................................................................................................................................................................. 8 Common abbreviations................................................................................................................................................................... 8
Chapter 1 Principles of hydrophobic interaction chromatography ................................................. 9
Hydrophobic interaction chromatography in theory .............................................................................. 10
The role of water............................................................................................................................................................................. 10 Protein structure .............................................................................................................................................................................. 10 Reversible interactions................................................................................................................................................................. 11 Steps in a HIC separation ............................................................................................................................................................ 11 Resolution........................................................................................................................................................................................... 14 Efficiency ............................................................................................................................................................................................ 15 Selectivity............................................................................................................................................................................................ 16
Selectivity and binding capacity .......................................................................................................................................................................... 16 Selectivity and salt selection.................................................................................................................................................................................. 16 Selectivity and the properties of a HIC medium ............................................................................................................................................ 17 Selectivity and elution...
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