Extraccion protwinas

Páginas: 222 (55397 palabras) Publicado: 28 de mayo de 2011
GE Healthcare

Hydrophobic Interaction and Reversed Phase Chromatography
Principles and Methods

Handbooks from GE Healthcare

Antibody Purification
Handbook 18-1037-46

Microcarrier cell culture The Recombinant Protein Handbook
Protein Expression and Simple Purification 18-1142-75 Principles and Methods 18-1140-62

2-D Electrophoresis
using immobilized pH gradientsProtein Purification
Handbook 18-1132-29

Principles and Methods 80-6429-60

Ion Exchange Chromatography & Chromatofocusing
Principles and Methods 11-0004-21

Hydrophobic Interaction and Reversed Phase Chromatography
Principles and Methods 11-0012-69

Affinity Chromatography
Principles and Methods 18-1022-29

Percoll
Methodology and Applications 18-1115-69

Gel FiltrationPrinciples and Methods 18-1022-18

Ficoll-Paque Plus
For in vitro isolation of lymphocytes 18-1152-69

Expanded Bed Adsorption
Principles and Methods 18-1124-26

GST Gene Fusion System
Handbook 18-1157-58

Hydrophobic Interaction and Reversed Phase Chromatography
Principles and Methods

Handbook 11-0012-69 AA 1

Contents
Introduction......................................................................................................................................... 7
Symbols .................................................................................................................................................................................................. 8 Common abbreviations................................................................................................................................................................... 8

Chapter 1 Principles of hydrophobic interaction chromatography ................................................. 9
Hydrophobic interaction chromatography in theory .............................................................................. 10
The role of water............................................................................................................................................................................. 10 Protein structure .............................................................................................................................................................................. 10 Reversible interactions................................................................................................................................................................. 11 Steps in a HIC separation ............................................................................................................................................................ 11 Resolution........................................................................................................................................................................................... 14 Efficiency ............................................................................................................................................................................................ 15 Selectivity............................................................................................................................................................................................ 16
Selectivity and binding capacity .......................................................................................................................................................................... 16 Selectivity and salt selection.................................................................................................................................................................................. 16 Selectivity and the properties of a HIC medium ............................................................................................................................................ 17 Selectivity and elution...
Leer documento completo

Regístrate para leer el documento completo.

Estos documentos también te pueden resultar útiles

  • extraccion
  • Extraccion
  • Extraccion
  • extraccion
  • Extracción
  • Extraccion
  • Extraccion
  • Extraccion

Conviértase en miembro formal de Buenas Tareas

INSCRÍBETE - ES GRATIS