Extraccion protwinas

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GE Healthcare

Hydrophobic Interaction and Reversed Phase Chromatography
Principles and Methods

Handbooks from GE Healthcare

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using immobilized pH gradientsProtein Purification
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Hydrophobic Interaction and Reversed Phase Chromatography
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Affinity Chromatography
Principles and Methods 18-1022-29

Percoll
Methodology and Applications 18-1115-69

Gel FiltrationPrinciples and Methods 18-1022-18

Ficoll-Paque Plus
For in vitro isolation of lymphocytes 18-1152-69

Expanded Bed Adsorption
Principles and Methods 18-1124-26

GST Gene Fusion System
Handbook 18-1157-58

Hydrophobic Interaction and Reversed Phase Chromatography
Principles and Methods

Handbook 11-0012-69 AA 1

Contents
Introduction......................................................................................................................................... 7
Symbols .................................................................................................................................................................................................. 8 Common abbreviations................................................................................................................................................................... 8

Chapter 1 Principles of hydrophobic interaction chromatography ................................................. 9
Hydrophobic interaction chromatography in theory .............................................................................. 10
The role of water............................................................................................................................................................................. 10 Protein structure .............................................................................................................................................................................. 10 Reversible interactions................................................................................................................................................................. 11 Steps in a HIC separation ............................................................................................................................................................ 11 Resolution........................................................................................................................................................................................... 14 Efficiency ............................................................................................................................................................................................ 15 Selectivity............................................................................................................................................................................................ 16
Selectivity and binding capacity .......................................................................................................................................................................... 16 Selectivity and salt selection.................................................................................................................................................................................. 16 Selectivity and the properties of a HIC medium ............................................................................................................................................ 17 Selectivity and elution...
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