Pallets

BUFFERED SALINE PEPTONE WATER
CAT Nº: 1406
FORMULA IN g/l
Sodium Chloride Casein Peptone 8.50 1.00 Disodium Phosphate Sodium Phosphate 0.044 0.023

Recommended as a primary solvent for serialdilution

Final pH 7.0 ± 0.2 at 25ºC

PREPARATION
Suspend 9.57 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minuteuntil complete dissolution. Dispense into appropriate containers and sterilize at 121°C for 15 minutes. The prepared medium should be stored at 2-8°C. The color is colorless. The dehydrated medium shouldbe homogeneous, free-flowing and very light beige in color. If there are any physical changes, discard the medium.

USES
BUFFERED SALINE PEPTONE WATER is a non inhibited medium, therefore allowingan easy recovery of stressed microorganisms. Buffered Saline Peptone Water is rich in nutrients and produces high recovery of damaged cells and intensifies the growth of microorganisms. A featurecommon to all selective media is that sublethally injured organisms are not generally detected and therefore a recovery step must be included in examination procedures. Changes in pH may damage bacteriagrowth. Buffered Saline Peptone Water maintains a high pH via the Phosphate buffer system and allows the repair of injured cells which are sensitive to low pH. Casein peptone provides nitrogen,vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance.

MICROBIOLOGICAL TEST
The following results were obtained inthe performance of the medium from type cultures after incubation at a temperature of 35ºC ± 2ºC and observed after 18-24 hours.
Microorganisms Growth Good Good Good

Escherichia coli ATCC 25922Salmonella typhimurium ATCC 14028 Staphylococcus aureus ATCC 25923

BIBLIOGRAPHY
Standard Methods for the Examination of Water and Wasterwater 15a edition, 1980 Juven, Cox Bailet, Rhomson, Charles...