traduccion western bloot

To compare the wester blotting technique to the ELISA
To observe the use of the western blotting technique to test for HIV
To distinguish between antigens and antibodies
Southern blotting was developed by ED Southern in 1975 to indentify DNA, A variation of this directional theme. Western blotting, another variation that identifies proteins, is named after the same convertion
Westernblotting uses an electrical current to separate proteins in the basis of their size and charge. This technique uses gel electrophoresis it the separate the proteins in a gel matrix. available
Because the resulting gel is fragile and would be difficult to use in further tests, the proteins are then transferred to a nitrocellulose membrane. The original WESTERN blotting technique used blotting(diffusion) to proteins, but electricity is also used now for the transfer of the proteins to nitrocellulose strip. These strips are commercially available, eliminating the need for the electrophoresis and transfer equipment. In this activity you will begin the procedure after the HIV antigens have already been transferred to nitrocellulose and cut into strips.
Wester blotting is also known asimmunoblotting because the proteins that are transferred, or blotted, onto the membrane are later treated with antibodies- the same procedure used in the indirect enzyme-liked immunosorbent assay ELISA . The ELISA Is considered enzyme linked because an enzyme is chemically linked to an antibody in both the direct and indirect version of the test. Immunosorbent refers to the fact that either antigens orantibodies are being adsorbed (stuck) to plastic. If the test is designed to detect an antigen or antigens, it is a direct ELISA because it is directly looking for the foreign substance. An indirect ELISA is designed to detect antibodies that the patient has made against the antigen.
Similar to the secondary antibodies used in the indirect ELISA technique, the secondary antibodies in the westernblot have an enzyme attached to them, allowing for the use of color to detect a particular protein. The secondary antibody binds to the constant region of the primary antibody found in the patient s sample. The main difference between these techniques is that the ELISA technique uses a well that corresponds to mixture of antigens, and the western blot has a discrete protein band that represents thespecific antigen that the antibody is recognizing LIKE HIV LYME DISEASE CAN ALSO BE DETECTED WITH THE WESTERN BLOT TECHNIQUE.
THE INITIAL TEST for HIV is the ELISA, which is less expensive and easier to perform than the Western blot .The Western blot is used as confirmatory test after appositive ELISA because the ELISA is prone to false-positive results. The bands from a positive Western blotare from antibodies binding to specific proteins and glycoprotein from the human immunodeficiency virus. A positive result from western blot is determined by the presence of particular proteins bands.
Washing buffer
Developing buffer secondary antibody conjugated with an enzyme
Rocking apparatus
Nitrocellulose strip
Troughs
Tray
Biohazardous waste disposalPara comparar la técnica de wester blot para la prueba ELISA
Para observar el uso de la técnica de western blot para detectar el VIH
Para distinguir entre antígenos y anticuerpos
Southern Blot fue desarrollado por ED Southern en 1975 a indentify ADN, una variación de este tema direccional. Western Blot, otra variación que identifica lasproteínas, se nombra después de la conversión misma
Western Blot utiliza una corriente eléctrica para separar las proteínas en función de su tamaño y carga. Esta técnica utiliza la electroforesis en gel se separan las proteínas en una matriz de gel. disponible

Debido a que el gel resultante es frágil y sería difícil de utilizar en pruebas adicionales, las proteínas se transfieren a una membrana de...