Hidrolisis enzimatica

Project Number DDB-2012

Enzymatic Hydrolysis of Cellulosic Biomass for the Production of Second Generation Biofuels
A Major Qualifying Project Report Submitted to the faculty of

Worcester Polytechnic Institute
In partial fulfillment of the requirements for the Degree of Bachelor of Science
By ____________________

Alexander Demers
___________________

Richard Doane__________________

Scott Guzman
__________________

Ryan Pagano
Date: May 1, 2009 Approved:

________________________

________________________

William M. Clark, PhD
Project Advisor

David DiBiasio, PhD
Project Advisor Project Number DDB-2012

Enzymatic Hydrolysis of Cellulosic Biomass for the Production of Second Generation Biofuels
Table of Contents
Abstract:................................................................................................................................................. 3 Introduction ............................................................................................................................................ 4 Background............................................................................................................................................. 6 What are Biofuels? .............................................................................................................................. 6 Cellulosic vs. Fossil Fuels .................................................................................................................. 6 What isCellulose?............................................................................................................................ 8 Sources of Cellulose ......................................................................................................................... 8 Why is Enzymatic Hydrolysis Important?.............................................................................................. 9 Alternative to petroleum products................................................................................................. 10 Highly renewable resource ............................................................................................................ 10 Significant breakthroughs/developments....................................................................................... 10 Reaction Pathway.......................................................................................................................... 12 Methodology......................................................................................................................................... 13 Materials used ................................................................................................................................... 13Equipment......................................................................................................................................... 15 Experimental Analysis........................................................................................................................ 17 Apple pomace pretreatment............................................................................................................. 18 Hydrolysis Step .................................................................................................................................. 18 Results: ................................................................................................................................................. 20 Discussion............................................................................................................................................. 26 Conclusion:............................................................................................................................................ 27 Recommendations ................................................................................................................................ 28...