Ion exchange chromatography principles and methods

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Ion Exchange Chromatography
Principles and Methods

18-1114-21 Edition AA

Ion Exchange Chromatography
Principles and Methods

ISBN 91 970490-3-4

Contents
1. 2. Introduction ............................................................................................9 Ion exchange chromatography .........................................................10
The theory of ionexchange .................................................................10 The matrix.....................................................................................11 Charged groups .............................................................................13 Resolution in ion exchange chromatography .................................13 Capacity factor........................................................................15 Efficiency.................................................................................16 Selectivity.................................................................................17 Capacity ........................................................................................18

3.

Product Guide.......................................................................................20
MonoBeads ...................................................................................20 MiniBeads .....................................................................................20 SOURCE .......................................................................................20 Sepharose High Performance ion exchangers.................................21 Sepharose Fast Flow ion exchangers ..............................................21 Sepharose Big Beads ion exchangers...............................................21 STREAMLINE ion exchangers ......................................................21 DEAE Sepharose CL-6B and CM Sepharose CL-6B .......................22 DEAESephacel..............................................................................22 Sephadex ion exchangers ...............................................................22 Bulk quantities...............................................................................22 Equipment.....................................................................................22

4.

MonoBeads and MiniBeads..............................................................23 MonoBeads.....................................................................................23
Properties ......................................................................................24 Chemical stability ....................................................................24 Physical stability......................................................................25 Flow rate..................................................................................27 Capacity ..................................................................................27 Recovery..................................................................................28 Reproducibility ........................................................................29 Availability....................................................................................30 MiniBeads .......................................................................................30 Properties ......................................................................................31 Chemical stability ....................................................................31 Physical stability......................................................................32 Reproducibility ........................................................................33 Availability ....................................................................................33

2

5.

SOURCE ................................................................................................34
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