Tricomonas

Giardia/Cryptosporidium Chek is an immunoassay developed to allow the simultaneous qualitative detection of Giardia and Cryptosporidium antigens in fecal specimens. It is a microtiter plateenzyme-linked immunosorbent assay (ELISA) that detects both Giardia and Cryptosporidium cyst antigens from fecal specimens. Positive results of the test signify the presence of either Giardia orCryptosporidium or the presence of both pathogens. It should be noted, however, that the test cannot distinguish between the two pathogens.
One hundred thirty-six fecal samples, of both diarrheal and nondiarrhealspecimens, were tested at the International Centre for Diarrheal Disease Research, Bangladesh. As a qualitative standard of measurement of the presence/absence of either or both of the antigens,individual ELISAs for Giardia and Cryptosporidium, the Giardia II test and the Cryptosporidium II test (TechLab), were used as the gold standard. All specimens with discrepant results were retested by theGiardia II test and the Cryptosporidium II test and by PCR tests. If the specimen was positive with the Giardia II test and/or the Cryptosporidium II test and further confirmed by the PCR tests, thespecimen was considered a true positive.
The Giardia/Cryptosporidium Chek assay was used in accordance to the manufacturer's directions. Four hundred microliters of diluent was added to 100 μl offecal sample in a tube and was mixed thoroughly. One hundred microliters of diluent and 50 μl of the mixture were added to the microassay plate and was left at room temperature for incubation for 1 h. Thewells were washed five times using the wash solution provided, and a drop (50 μl) of conjugate was added to each well, which was incubated at room temperature for 30 min, followed by washing.Subsequently, 2 drops (100 μl) of substrate was added to each well. After 10 min of incubation at room temperature, 1 drop (50 μl) of stop solution was added to each well. The plate was read using an ELISA...