Desnaturalizacion De Proteinas
Department of Environmental Engineering, Kasetsart University, 50 Phaholyothin Road, Chatuchak, Bangkok 10900, Thailand. Fax +662-5790730 * Email: fengccc@ku.ac.th 2 Integrated ResearchSystem for Sustainability Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan 3 Environmental Science Center, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan
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Abstract This paper presents a case study on application of photosynthetic bacteria wastewater treatment system to recover organic carbon in wastewater in the form of photosynthetic bacterial cells. Innoodle production process, the wastewater was mainly produced during rice soaking and rinsing processes containing starch as its main constituent. For carbon recovery purpose, wastewater was applied to laboratory photosynthetic wastewater treatment system where carbon could be transformed into protein in purple non-sulfur or purple sulfur bacterial cells. The system has shown to be effective for thetreatment of starch wastewater and cultivation of photosynthetic bacteria when operated at hydraulic retention time of 6 and 10 days.
1. Introduction The application of photosynthetic bacteria to wastewater treatment is one of the attractive methods since the microorganisms are widely available in nature and capable of removing organic substances even under high organic load (Hiraishi et al.,1989) especially in sunlight intensive area like tropical countries. The treatment will require minimum additional energy apart from natural sunlight and the treatment cost can be substantially reduced. They are applicable to organic compounds (Madigan et al., 2000) and wastewater from various industries (Noparatnaraporn et al., 1986). By introducing this system, it might be possible to develop ahigh-efficiency treatment process without odorous gas production. The proposed system (Figure 1) consists of two ponds connected in series. In the first pond, it is expected that acidogenic bacteria consume organic matter in wastewater and photosynthetic bacteria grow with their metabolite. Then the following aerobic fishpond is expected to have a high fish yield. Moreover the by-product of thetreatment, the photosynthetic bacteria cells, has high nutrition such as protein and vitamin (Kobayashi and Tchan, 1973; Getha et al., 1998; Banjaree et al., 2000) which is a good alternative for fish feed.
Wastewater Organic matters
Anaerobic photosynthetic treatment pond Acidogens
Fishpond Aerobic stabilization pond Plankton
Treated water
PnSB
Fatty acids, alcohols
Figure 1.Process flow of photosynthetic bacteria pond treatment system.
2. Wastewater from noodle production Typical production of rice noodle includes broken rice and water as two main raw materials for production of unfermented rice noodles. It involves washing and soaking of rice, pasting the rice and making the starch slurry. For thick noodle processing, the slurry is then used in sheeting prior tocooking and drying step. For thin noodle processing, the sheeted slurry is cooked and dried in an oven before cutting process. The processing of vermicelli noodle is slightly different from thick and thin noodle. The starch slurry is passed through a filter press, yielding semi-dry starch solids. The starch solids are steamed before being extruded to form vermicelli noodles. The production of ricenoodles produces wastes at various stage, i.e. raw material preparation, production, packaging and process line cleaning. Among these, wastewater is mainly produced during rice soaking/rinsing and cleaning of pasting tank. It contains starch as main constituents with high BOD and suspended solids. 3. Experimental system Laboratory scale reactors (3-L working volume) simulating photosynthetic...
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