Adenovirus e1a y tumor de ewing

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© 2000 Nature America Inc. • http://medicine.nature.com

LETTERS TO THE EDITOR

Adenovirus E1A and Ewing tumors
In the December 1999 issue of Nature Medicine, Melot and Delattre1 and Kovar2, challenged the Sanchez-Prieto et al. September 1999 article3 proposing that the adenoviral E1A gene induces the Ewing tumor fusion transcript EWS–FLI1. Here Sanchez-Prieto et al. respond…Ed.Sanchez-Prieto et al. reply—Both letters question the association between E1A and EWS–FLI1 by studying HEK293 cells using RT–PCR. HEK293 cells derive from human embryonic kidney cells immortalized by adenoviruses many years ago4. Since then, several different cell subclones of 293 and derived cells have been generated (HEK293, 293-T, Phoenix and Bosc23, for example). In our experiments, we studied HEK293cells (obtained directly from the American Type Culture Collection (ATCC)), HEK293-T cells (obtained from S. Gutkind, National Institutes of Health), HEK293 cells cultured for a long time in our laboratory, and the Phoenix and BOSC23 cells. We were able to detect fusion transcripts of types 1 and 2 (always with nested PCR) in some subclones of 293 cells: type 2 in our HEK293 cells, type 1 in theHEK293 from the ATCC, and an almost-undetectable fusion transcript in the HEK293-T. The results were variable and depended on many parameters, including PCR conditions, RNA extraction, the primer used to generate cDNA (FLI1-specific was better than random) and the cell lines studied. We detected fusion transcripts much more efficiently in the Bosc23 and Phoenix cells. Therefore, in response to Melotand Delattre and Kovar, we can only say that detection of fusion transcript EWS–FLI1 in HEK293 cells may depend on the subclones studied and assay conditions, and that an association between a high level of E1A protein and the EWS–FLI1 fusion product is not always found. We agree with Melot and Delattre that the different sizes of EWS–FLI1 proteins and cDNAs may be associated with differentretroviral infections in IMR90 cells. We have detected fusion transcripts of types 1 and 2 in HEK293 and derived cells. In any case, the 293 and derived cells are aneuploid and polyclonal, and we interpret this to mean that the different sizes of the EWS–FLI1
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fusion transcripts can be related to different subclones of 293 cells. Melot and Delattre also questioned our interpretation of ourfluorescent in situ hybridization (FISH) results using a cosG9 chromosome 22 cosmid (Fig. 3b in ref. 3). In fact, we incorrectly labeled this figure, which actually shows FISH with a centromeric probe of chromosome 11 and the Vysis LDI DiGeorge/Dual Color Probe, but not cosG9. Our original study used several probes for FISH, including those that recognize whole chromosomes 11 and 22, centromeric probes,cosG9, cos1d1 and the DiGeorge probe. This last probe contains a mixture of TUPLE1 (22q11.2) in orange and LSI AASA (22q13) in green. In cells with the translocation, we detected the probe marked in green (terminal region of chromosome 22) and the centromeric probe of chromosome 11 (Fig. 3b in ref. 3). We apologize for this mistake. In our paper, we emphasized that both IMR90 and HeLa E1A-expressingcells contain the fusion transcript EWS–FLI1 in a substantial number of cells. We have confirmed these results in new experiments (always after selection of E1A-expressing cells). Kovar mentions that they have studied the HeLa cell line 70 hours after transient transfection, with a transfection efficiency of only 2% of the cells. We think that the EWS–FLI1 fusion product is unlikely to be detectedin those conditions. Kovar did not find E1A sequences in any of either the 27 Ewing tumor cell lines or the 19 primary Ewing tumors he examined. We have subsequently studied three Ewing tumor cell lines (RD-ES, CADO and MHH-ES-1) and tumors from 32 patients with Ewing tumors (14 with EWS–FLI1 type 1 fusion, 15 with EWS–FLI1 non-type1 fusion and 3 with EWS–ERG fusion), normal tissue of these...
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